| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 가을 (10/13 ~ 10/14, 제주 ICC) |
| 권호 | 30권 2호 |
| 발표분야 | 기능성 고분자 |
| 제목 | Surface-Initiated Growth of Poly d(A-T) by Taq DNA Polymerase |
| 초록 | We report surface-initiated d(A-T) polymerization by Taq DNA polymerase as a method for constructing DNA-tethered surfaces using an enzyme. The enzymatic polymerization was conducted successfully via two steps: tethering of oligo d(A-T)s onto the surface presenting carboxylic acids by amide coupling and surface-initiated polymerization using Taq DNA polymerase. In this enzymatic polymerization process, the design and construction of carboxylic acid-presenting surfaces was found to be an important factor: DNA growth did not occur on the gold surface coated only with the self-assembled monolayer (SAM) of 16-mercaptohexadecanoic acid (MHDA), but effectively proceeded on the surfaces presenting mixed SAMs of MHDA and 1-pentadecanethiol (PDT). The coupling of oligo d(A-T)s and the subsequent DNA polymerization reaction were characterized by polarized infrared external reflectance spectroscopy, ellipsometry, X-ray photoelectron spectroscopy, and atomic force microscopy. 그림 1. Schematic representation for surface-initiated poly d(A-T) growth by Taq DNA polymerase. |
| 저자 | 지영식, 정영환, 이준성, 조우경, 최인성 |
| 소속 | 한국과학기술원 |
| 키워드 | Surface-Initiated Polymerization; DNA; Taq DNA Polymerase |
