| 초록 |
Synthetic hydrogels have been extensively investigated as artificial extracellular matrices(ECMs). Crucial challenges for such hydrogels are sustaining long-term cytocompatible encapsulation and providing appropriate cues for spatio-temporal control of the cells. Here, we report in situ supramolecularly assembled and modularly modified hydrogels for long-term engineered mesenchymal stem cell(eMSC) therapy using cucurbit[6]uril conjugated hyaluronic acid(CB[6]-HA), diaminohexane conjugated HA(DAH-HA). The eMSCs producing enhanced green fluorescence protein(EGFP) remained alive and emitted the fluorescence within CB[6]/DAH-HA hydrogels in mice for more than 60 days. Furthermore, the long-term expression of mutant interleukin-12(IL-12M) by eMSCs within the hydrogels resulted in effective inhibition of tumor growth with a significantly enhanced survival rate. Taken together, our findings confirm the feasibility of supramolecular HA hydrogels as 3D artificial ECMs for cell therapies. |
