| 학회 |
한국고분자학회 |
| 학술대회 |
2009년 가을 (10/08 ~ 10/09, 광주과학기술원 오룡관) |
| 권호 |
34권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
MMPs-cleavable electrospun nanofibers for gene delivery |
| 초록 |
Nano-inspired polymeric non-woven meshes were prepared by electrospinning for potential application of efficient gene delivery at wound sites. A matrix metalloproteinase (MMP) family is a hydrolytic enzyme highly expressed in diabetic ulcers to facilitate cellular migration during wound healing process. In this study, poly(ε-caprolactone)-poly(ethyleneglycol)[PCL-PEG] block copolymer was electrospun to non-woven and nanofibrous structures. In order to obtain controlled release of therapeutic DNA from nanofibrous scaffolds, a MMPs-specific peptide, DGPLGVC was employed as a MMP-cleavable linker between electrospun nanofibers and cationic polymers for gene delivery vehicles. For conjugation of PEI, PEI was activated with SPDP and peptide-immobilized nanofibers were subsequently mixed. The average diameter was approximately 890nm. PEI density on the PEI-nanofiber was 0.384nmol/mg, suggesting that 99.2% of the exposed amines participated in the reaction. |
| 저자 |
김혜성1, 양건호2, 남궁란3, 최지숙1, 박신영1, 정현아1, 유혁상1
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| 소속 |
1강원대, 2성균관대, 3포항공과대 |
| 키워드 |
MMPs; Electrospun nanofiber; gene delivery
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| E-Mail |
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