| 학회 |
한국고분자학회 |
| 학술대회 |
2012년 가을 (10/11 ~ 10/12, 창원컨벤션센터) |
| 권호 |
37권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Spurred Gene Transfer through Stimulating Osmotic Fragility of Endosome by Polyxylitol-based Transporter |
| 초록 |
Endosomal escape is one of the most important processes in the efficient non-viral gene delivery system and far much efforts have been taken to enhance this step. Here, we designed the new non-viral vector, polyxylitol-based transporter(PXT) having enhanced endosomal escape through stimulating osmotic fragility of endosome. PXT was synthesized through Michael addition reaction between xylitol diacrylate as a crosslinker and low molecular weight PEI(1.2K) and it showed higher cell viability and transfection efficiency than those of PEI 25kDa. The small amount of xylitol integrated in PXT(4% w/w) made one-third of osmotic pressure in PXT and this made endosome more swell and fragile, thus yielding pDNA at least 10-fold more released into cytosol and 4-fold more localized to nucleus compared with PEI. Probably this mechanism will be also valid to post-mitotic, differentiated C2C12 cells and we expect that the PXT might be a efficient gene carrier through this endosome-escaping mechanism. |
| 저자 |
이원석1, 김유경2, Qiankun Zhang3, 박태은4, 강상기5, 최윤재6, 조종수1
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| 소속 |
1서울대, 2Department of Pharmaceutics, 3School of Pharmacy, 4China Pharmaceutical Univ., 5Nanjing 210009, 6China |
| 키워드 |
Non-viral gene delivery; PXT; endosomal escape; osmotic fragility
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| E-Mail |
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