| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 가을 (10/30 ~ 11/01, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
23권 2호 |
| 발표분야 |
포스터_생물공학 |
| 제목 |
Fluorescence detection of melamine based on its functions as fluorescence enhancer of DNA-AgNCs and Hg(II)-scavenger |
| 초록 |
A new method has been developed for the simple, fluorescence, turn-on detection of melamine, which utilizes DNA-templated silver nanoclusters as a key component. In the sensor, melamine exhibits the dual functions: one is to enhance the fluorescence signal of DNA-AgNCs by its interaction with thymine residues in DNA template, and the other is to prevent Hg(II)-induced fluorescence quenching of DNA-AgNCs via its coordination with Hg(II). These consequently enable the sensitive and selective detection of melamine. By exploiting such features of melamine, we increased the fluorescence response up to 360%, compared to the previous counterpart that relies on DNA-AgNCs only, and successfully determined melamine down to ca. 49 nM, a value that is 400 times lower than the safety level of 20 μM set by the US Food and Drug Administration. In addition, it was confirmed that the proposed approach works fine even in the real milk samples without any additional pre-treatment steps. |
| 저자 |
김지민, 정세한, 권우영, 황성현, 김석준, 차병석, 박기수
|
| 소속 |
건국대 |
| 키워드 |
Biosensor; DNA-templated silver nanoclusters; fluorescence; melamine; Hg(II)-scavenger
|
| E-Mail |
|
