| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 가을 (11/03 ~ 11/05, 대구 엑스코(EXCO)) |
| 권호 |
25권 2호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Sample preparation system for pathogen detection for biological contamination of surface using graphene oxide-coated bead |
| 초록 |
Analysis of biological contamination in surfaces is important to public health. As the current method uses cotton swabs for sampling, it is difficult to cover a large area, and it has low sensitivity. To overcome this issue, surface sampling was conducted with a half A4 sized wipe. Pathogens on the surface were collected on the wipe and transferred into the solution by a gentle vortexing. Pathogens in the solution were concentrated on the graphene oxide coated micro-sized beads. The nucleic acid of the pathogen was extracted by using the bead beating method and it was quantified by polymerase chain reaction. As a result, it can detect 102 cfu(pfu)/m2 of pathogens within 1.5 hours. |
| 저자 |
이운광, 백창윤, 유현진, 최문영, 류봉거, 강가동, 민준홍
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| 소속 |
중앙대 |
| 키워드 |
Surface sample preparation; Graphene oxide; Polymerase chain reaction
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| E-Mail |
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