| 초록 |
The aim of this study was to develop a new gene carrier consisting of water-soluble chitosan (SC), bioreducible material, and PEI. We found that bioreducible material and PEI modified soluble chitosan (SCBP) can be deliver faster gene into nucleus of mammalian cell. Gelretardation assay showed that pDNA was completely condensed by SCBP, and protected from DNase. Also, Its transfection was increased by enhanced proton sponge effect with modified PEI, and bioreducible material was dissociated by glutathione of cytosol into cell. In addition, its cellular uptake was investigated by fluorescent microscope. Characterization of SCBP was analysed by FT-IR, 1H-NMR, DLS and TEM studies. Moreover, Its cytotoxicity was confirmed by MTT assay. These results suggest that SCBP can be apply as a carrier of gene delivery. This work was supported by National Research Foundation of Korea (NRF) grant funded by the Ministry of Science, ICT & Future Planning. (No. NRF-2014R1A2A1A10053027). |
