| 학회 |
한국화학공학회 |
| 학술대회 |
2016년 가을 (10/19 ~ 10/21, 대전컨벤션센터) |
| 권호 |
22권 2호, p.1836 |
| 발표분야 |
생물화공 |
| 제목 |
Efficient self-assembled microRNA delivery system consisting of cholesterol-conjugated micreRNA and pegylated polycationic polymer |
| 초록 |
MicroRNA (miR), a key molecule of endogenous RNA interference (RNAi), is promising as a therapeutic agent to diseases. In vivo delivery of miR, however, is a major obstacle as its polyanionic nature and vulnerability to serum render it difficult to reach targeted lesion. To overcome these hurdles, we present a self-assembled miR delivery system consisting of cholesterol-conjugated miR (chol-miR) and pegylated polycationic polymer, poly ethylene glycol grafted poly ethylene imine (PEG-g-PEI). In our study, nanosized complexation of miR with PEI, suitable to effective protection and delivery of miR in vivo, was stably achieved by PEG grafting. Hydrophobicity of cholesterol conjugated to miR assisted structural solidity in PEG-g-PEI/chol-miR, avoiding undesirable loss of miR. Here we report preparation and analysis of PEG-g-PEI and self-assembled PEG-g-PEI/chol-miR complex with a model cell line, Hs746T (human metastatic gastric cell line). In conclusion, our miR delivery system with PEG-g-PEI/chol-miR showed considerable potential for the effective in vivo delivery of miR. |
| 저자 |
문병걸1, 한승민1, 장은지2, 강병훈1, 기지선1, 손혜영2, 최유나2, 함승주1, 허용민2
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| 소속 |
1연세대, 2Severance Biomedical Science Institute (SBSI) |
| 키워드 |
바이오칩/나노바이오공학 |
| E-Mail |
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| 원문파일 |
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