| 학회 |
한국고분자학회 |
| 학술대회 |
2010년 봄 (04/08 ~ 04/09, 대전컨벤션센터) |
| 권호 |
35권 1호 |
| 발표분야 |
세포기능조절 고분자(의료용 고분자 부문위원회) |
| 제목 |
Regeneration of Biodisc Using Annulus Fibrosus Cell on Hyaluronic Acid Loaded PLGA Scaffold, in vivo |
| 초록 |
Poly(lactide-co-glycolide)(PLGA) and hyaluronic acid (HA) has been widely used as biocompatible scaffold materials to regenerate tissue. In this present study, we fabricated microporous PLGA and HA loaded PLGA scaffolds by salt-leaching and then penetrating method. AF cells were seeded in HA/PLGA scaffold, and then PLGA and HA/PLGA for the in vivo study were implated in 4 week old nude mice. All scaffolds were characterized using a scanning electron microscope(SEM) / Reverse transcription polymerase chain reaction(RT-PCR) was assessed to measure mRNA expression and glycosaminoglycan(GAG) quantity from annulus fibrosus cells in scaffolds. It was proven by higher specific mRNA expression in the HA-PLGA scaffold than PLGA alone. In addition, annulus fibrosus strongly produced sGAG in HA/PLGA. This result indicates that HA/PLGA scaffold is useful for intervertebral disc regeneration . This research was supported by WCU(R31-20029) |
| 저자 |
안우영, 최진희, 이동원, 강길선
|
| 소속 |
전북대 |
| 키워드 |
PLGA; Hyaluronic acid (HA); Annulus Fibrosus
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| E-Mail |
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