| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 가을 (10/13 ~ 10/14, 제주 ICC) |
| 권호 | 30권 2호 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Novel polymer/nano-bioceramic composite scaffold for bone tissue engineering |
| 초록 | Objectives: Biodegradable polymer/bioceramic composite scaffolds for bone tissue engineering can overcome the limitations of conventional ceramic bone substitutes. However, conventional methods (e.g., solvent casting and particulate leaching (SC/PL)) to fabricate polymer/bioceramic composite scaffold use organic solvents, which might be harmful to cells or tissues and hinder the exposure of the ceramics to the scaffold surface. In this study, a novel polymer/nano-bioceramic composite scaffold with highly exposed bioceramics to the scaffold surface was developed for efficient bone tissue engineering. Methods: Poly(D,L-lactic-co-glycolic acid)/nano-hydroxyapatite (PLGA/HA) composite scaffolds were fabricated by gas foaming and particulate leaching (GF/PL) method without the use of organic solvent. Both scaffolds were seeded with rat calvarial osteoblasts and cultured in vitro or subcutaneously implanted into athymic mice for 8 weeks. Results: The GF/PL method exposed HA nanoparticles to the scaffold surface at significantly higher extent than conventional SC/PL method. The GF/PL scaffolds showed interconnected porous structures without skin layer, and exhibited enhanced mechanical properties in comparison with SC/PL scaffolds. The GF/PL scaffolds exhibited significantly higher cell growth, alkaline phosphatase activity, and mineralization compared to the SC/PL scaffolds in vitro. Histological analyses and calcium content quantification of the retrieved tissues 8 weeks after implantation showed more extensive bone formation on the GF/PL scaffolds than SC/PL scaffolds. Compared to the SC/PL scaffolds, the enhanced bone formation on the GF/PL scaffolds may result from the higher exposure of HA nanoparticles to the scaffold surface, which allows for direct contact with transplanted cells and would stimulate the cell proliferation and osteogenic differentiation. Conclusions: The polymer/bioceramic composite scaffolds fabricated with the novel GF/PL method could enhance bone regeneration compared with conventional SC/PL scaffold. |
| 저자 | 김상수1, 박민선2, 최차용1, 김병수2 |
| 소속 | 1서울대, 2한양대 |
| 키워드 | bone; tissue engineering; scaffold |
