| 초록 |
This work reportsthe immobilization ofmonomer, dimer, trimer, tetramer (multimer) protein G onto amine functionalized magnetic silica nanoparticles for self-orientation and immobilization of antibody. The surface of the magnetic silica nanoparticles was modified with 3-aminopropyl-trimethoxysilane (APTMS) to chemically link to multimer protein G. The conjugation of amine on the MNPs to cysteine tag in multimer protein Gs was performed using a sulfo-SMCC coupling procedure. The binding efficiencies of mono-, di-, tri- and tetramer were 77%, 51%, 43%, 20% respectively. The bound of multimer protein Gs was characterized by using ATR FT-IR, and transmission electron microscopy (TEM), and UV-VIS spectrophotometer test. Afterward mouse IgG, model antibody, was immobilized on the nanoparticle through multimer protein Gs. The multimer protein Gs bound magnetic silica nanoparticles was found to be a more suitable tool for a sensitive immunosensor. |
