| 초록 |
Non-viral vectors for gene therapy have been increasingly proposed as safer alternatives to viral vectors. As a novel gene carrier, we synthesized poly(diallyl amine)(PdAA) by polymerization of diallylamine monomer with V-50 as a radical initiator at 60℃ for 24h under a nitrogen atmosphere. And to increase water solubility and biocompatibility, PdAA with different degrees of glycolylation was compared to non-modified PdAA. The obtained polymers were characterized by 1H-NMR spectroscopy. The copolymer/DNA complex were characterized by measuring particle sizes, zeta potential and AFM. And we performed in vitro cytotoxicity test and transfection experiment. The prepared PdAA and degree of glycolylation of PdAA were confirned with 1H-NMR. The complete polymer/DNA complex was formed at the N/P ratio of 1 and the complex to DNaseⅠ resistance was obtained. In PdAA, the particle sizes decreased with an increase of N/P ratio, but them of glycolylated PdAA were not changed regardless of N/P ratio. And above N/P ratio 5, zeta potential of glycolyated PdAA/DNA complexes were not influenced by N/P ratio contrary to PdAA. Cell viability increased with an increase of degree of glycolylation. Also, transfection efficiency was influenced by degree of glycolylation, and in case of PdAA, the transfection efficiency was higher than PEI 25K in 293T whereas it was similar with PEI 25K in HeLa. The transfection efficiency of glycolylated PdAA was decreased with an increase of glycolyation owing to the increased hydrophilicity.
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