| 학회 |
한국화학공학회 |
| 학술대회 |
2014년 가을 (10/22 ~ 10/24, 대전 DCC) |
| 권호 |
20권 2호, p.1556 |
| 발표분야 |
생물화공 |
| 제목 |
Self-assembly of Cellulosome-based Biosensor with a Cascaded Signal Amplification for Utilizing as Enzyme and Whole-cell Biosensor |
| 초록 |
A simple and novel promising strategy is proposed for a biosensor with high sensitivity due to signal amplification based on self-assembled protein interactions. To construct the self-assembled biosensor, a cellulosome system, comprising Type I and Type II dockerin-cohesin interactions with different specificity, from the anaerobic Clostridia bacterium was applied. To design the dockerin-cohesin interaction based biosensor system, we constructed a self-assembled unit (SA unit) containing a type I cohesin module was fused to a dockerin type II module at the N-terminus and two type II cohesin modules at the C-terminus. Additionally, as optical biosensors, a green fluorescence protein (GFP) that was fused to a type I dockerin module was used as the output detection signal. The self-assembled biosensor was highly sensitive and achieved detection levels of 128.1-fold over the control. These results suggest that the designed, self-assembled biosensor system may be a promising strategy for utilizing an enzyme and whole-cell based biosensor. |
| 저자 |
현정은, 강대희, Tran The Thien, 한성옥
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| 소속 |
고려대 |
| 키워드 |
Biosensor; Cellulosome; Fluorescent protein; Self-assembly; Signal amplification
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| E-Mail |
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| 원문파일 |
초록 보기 |
