| 초록 |
Protein Immunohistochemistry is widely used in medical science. Integration of the probe and target biomolecules generally results from one or more non-covalent interactions. Therein nonspecific binding with similar interaction performance as specific binding in protein immunohistochemistry can produce high background noises, resulting in inconclusive target elucidation that hinders interpretation. We were designed and used by our group as biomolecules substrates using a parallel plate rheometer. Results showed that the shear stress influenced interactions between biomolecules. By changing the shear stress, we are able to control the ratio of specifically-bonded biomolecules to non-specifically-bonded biomolecules. Interestingly, higher accuracy appeared at the high shear stress, where shear stress caused higher shear rate of reaction solution, which means nonspecific binding can be largely replaced by specific binding due to molecular physical interactions. Furthermore, some other kinds of biomolecules were also tested under their optimal conditions to have an overall conclusion. |
