| 학회 |
한국고분자학회 |
| 학술대회 |
2014년 봄 (04/10 ~ 04/11, 대전 컨벤션센터) |
| 권호 |
39권 1호 |
| 발표분야 |
의료용 고분자 부문위원회(초청-영어발표/포스터-국문발표 가능) |
| 제목 |
Bone regeneration with neuropeptide substance P coupled with self-assembling peptide nanofiber in a rat calvarial defect model. |
| 초록 |
In this study, bioactive KLD12(KLD12+KLD12-Substance P) was developed to mimic endogenous tissue healing process. PLA(Poly lactic acid)/β-TCP(beta-tricalcium phosphate)+bioactive KLD12 was applied to repair calvarial defect. The structures of KLD12, KLD12-SP, bioactive KLD12 were observed by TEM and CD spectra. Bioactive KLD12 nanofibers(5-10nm) were created in physiological conditions by forming β–sheet structure. In vivo MSCs recruiting ability of bioactive KLD12 was observed by using in vivo fluorescence imaging system I-VIS. It was demonstrated that the i.v. injected NIR-labeled hBMSCs were recruited to the scaffold containing bioactive KLD12. Bone tissue regeneration was evaluated by observing gross morphologies H&E and MT staining datas of 12w and 24w after surgery. From the results, it was considered that PLA/β-TCP+bioactive KLD12 composite construct enhance bone tissue regeneration without cell transplantation. |
| 저자 |
김수희1, 허우준2, 김지은1, 김수현1, 최태현2, 정영미1
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| 소속 |
1한국과학기술(연), 2서울대 |
| 키워드 |
calvarial defect; self-assembling peptides; mesenchymal stem cell recruiting
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| E-Mail |
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