| 초록 |
Design of an efficient fingerprint that detects homologous proteins at distant sequence identity has been a great challenge. We have developed a strategy to extract an ideal-like fingerprint with high specificity and sensitivity from a group of sequences related to a fold. The fingerprint was generated by selecting the overlapped conserved residues (OCR) from the conserved residues of homologous proteins obtained using independent three alignment methods, i.e. multiple sequence alignment, structure-based alignment, and alignment based on the interstrand hydrogen-bonds. The OCR-based fingerprints were tested on protein folds of various classes with discrete sequence similarities, and showed more than 90% detection efficiency for all the folds tested. Finally, the approach was employed in the computational screening of novel enzymes such as Cupredoxin proteins and Cytochrome P450 proteins from protein sequence database. |
