| 학회 |
한국화학공학회 |
| 학술대회 |
2014년 가을 (10/22 ~ 10/24, 대전 DCC) |
| 권호 |
20권 2호, p.1611 |
| 발표분야 |
생물화공 |
| 제목 |
Detection of Helicobacter Pylori DNA using by hydrofluoric acid immobilized Carbon Probe Electrodes 불소고정 탄소전극의 헬리코박터파이로리 진단 |
| 초록 |
This paper describes the sensitive detection of HF immobilized biosensor for the rapid quantification of Helicobacter Pylori bacterium DNA, which is related to human gastritis cancer and gastric ulcers. Here, PCR testing methods require complicated separation and amplification techniques. However, electrochemical methods are fast and sensitive. Here, only detection that can be done within 60 sec was performed; the experiment in this process of cyclic and square wave voltammetry as a normal method was examined using HF coated working probe, graphite counter and reference probe. Here, in optimum para conditions such as experimental amplitude, frequency, initial potential, incremental potential, accumulation times, and others were examined. Final results showing the value of the current peak was determined using high sensitive signals or low detection limits. It is applied to live cells by comparing the presence of helicobacter DNA. |
| 저자 |
한승엽1, 이수영2, 이 경3, 김태윤1, 김진성1, 황혜리1, 김신원1, 박태수1, 이승한1
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| 소속 |
1바이오센서(연), 2서울과학기술대, 3Fudan Univ. |
| 키워드 |
Helicobacter Pylori DNA; hydrofluoric acid; immobilized Carbon Probe Electrodes
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| E-Mail |
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| 원문파일 |
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