| 초록 |
Industrially, Klebsiella pneumoniae can consume glycerol as carbon source for production of value added chemicals such as 3-hydroxypropionate and 1,3-propanediol, and also known as exo-electrogens in the previous reports. Respiration in bacteria is mainly regulated by external electron acceptor, and significantly affect to internal cell redox states. Interestingly, various microorganisms such as electrochemically active strains, use metal ions and/or solid carbon electrode as terminal electron acceptor, thus it have been extensively used as biocatalyst for electricity generation in MFC. In this study, metabolic shift by the activation of electrode based-anaerobic respiration in microbial fuel cell was investigated using Klebsiella pneumoniae L17. To determine the metabolic shift, both mRNA and metabolite level analysis were conducted with metabolic pathway reconstruction. Electricity generation was found nearly 146μA in MFC. The results indicate that anaerobic electron excretion with electrode results in metabolic pathway shift, and it could provide a strategy to redirect toward reductive pathway in K.pneumoniae L17 in the fermentation process.
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