| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 |
21권 1호 |
| 발표분야 |
생체재료 |
| 제목 |
Enhancement of Vascular Formation of Endothelial Cells Co-cultured with Human Adipose-derived Mesenchymal Stem cells using electrospun fiber |
| 초록 |
Blood vessel is essential process for tissue regeneration to deliver oxygen and nutrients and maintain tissue metabolism. Here we developed a technique to reconstruct microvascular system using polycaprolactone (PCL) and gelatin based a nanofibrous structure and co-culture system. Using a facile and simple electrospinning process, we fabricated 3D mesh scaffold to support endothelial cells (ECs) sprouting along the electrospun nanofiber, and co-culture with adipose-derived mesenchymal stem cells (ADSCs) supporting greater sprouting of ECs. The vascular formation in our system showed more effective paracrine signal from ADSCs to assist vascular formation of ECs than influence of growth factor. Co-cultured ECs expressed higher level of vascular markers (PECAM-1, podocalyxin). We established a bioengineered 3D culture system to promote fast and functional vascular. Co-culture system in nanofibrous scaffold can be applied in the future for restoration of blood vessel. |
| 저자 |
국윤민1, 이강원2, 고원건1
|
| 소속 |
1연세대, 2서울대 |
| 키워드 |
Co-culture; Vasculature; Angiogenesis; Electrospinning; Mesenchymal stem cell
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| E-Mail |
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