| 초록 |
Due to environmental problem, oil-based industries should be replaced to renewable bio-based industries. Especially, C4 chemicals such as 1,4-butanediol, gamma-butyrolactone and tetrahydrofuran were very important chemicals in our daily life. In this article, we report development of metabolically engineered Manneheimia succiniciproducens strains capable of efficiently producing 4-hydroxybutyrate. Here we report development of a metabolically engineered strain of Mannheimia succiniciproducens that produces 4-hydroxybutyrate which is a C4 chemical precursor at high titer. The strain was constructed using Mannheimia succiniciproducens LPK7 with overexpressing 4-hydroxybutyrate synthesis enzyme which is Succinyl-CoA synthetase, CoA dependent succinate semialdehyde dehydrogenase and 4-hydroxybutyrate dehydrogenase. This system can be used for development of C4-chemicals in bio-platform. [This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries. (NRF-2012-C1AAA001-2012M1A2A2026556)] |
