| 학회 |
한국공업화학회 |
| 학술대회 |
2018년 가을 (10/31 ~ 11/02, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
22권 2호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Efficient production of 5-aminolevulinic acid through re-distribution of metabolic carbon flux |
| 초록 |
5-aminolevulinic acid (ALA) has potential in clinical application as we want to engineer E.coli to produce this chemical. The aim of this study was precise carbon flux optimization for high ALA production in Escherichia coli expressing the ALA biosynthetic pathway. Initially, we can set first strategy that is overexpressing genes hemA and hemL with strong promoters and synthetic 5’-untranslated regions (5’-UTRs). Then, the tricarboxylic acid (TCA) cycle was blocked to force carbon flux toward the ALA production pathway by deletion of sucA. Although the resulting strain showed a severe metabolic imbalance and low ALA production, further precise tuning of carbon flux to the glyoxylate cycle by varying the transcriptional strength of aceA led to substantially improved cell growth and ALA production. Thus, this fine tuning of the glyoxylate cycle in a quantitative manner should also enable efficient production of other value-added products derived from the TCA cycle. |
| 저자 |
조민지1, 노명현1, 임현규1, 박성훈2, 서상우3, 정규열1
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| 소속 |
1POSTECH, 2UNIST, 3서울대 |
| 키워드 |
ALA; 5-aminolevulinic acid; Glyoxylate shunt
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| E-Mail |
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