| 학회 | 한국화학공학회 |
| 학술대회 | 2011년 봄 (04/27 ~ 04/29, 창원컨벤션센터) |
| 권호 | 17권 1호, p.315 |
| 발표분야 | 분리기술 |
| 제목 | Simulation of Immunoglobulin Yolk Purification by Simulated Moving Bed |
| 초록 | IgY (Immunoglobulin Yolk) is a specific antibody in egg yolk, and it protects human body from virus and antigen. There are a lot of egg yolk components such as lipoprotein and protein. To separate IgY, HPLC (High Performance Liquid Chromatography) and precipitation were used in a batch mode and SMB (Simulated Moving Bed) was adopted for continuouspurification of yolk proteins. IgY and other proteins in yolk were separated by using three-zone SMB chromatography. Before performing SMB experiments, batch chromatography and PIM (pulse input method) were performed to find operation parameters and adsorption isotherms. The results of batch chromatography were compared with simulated results using Aspen chromatography. To find the most suitable separation condition in SMB chromatography, simulations in m2-m3 plane on the triangle theory were carried out. m2 = 0.18, m3 = 1.0 and △t = 419 s are the best conditions for the highest purity of IgY.With this operating parameters (flow rate in three zone and switching time), the purity of raffinate results in 98.39 % from Aspen chromatography simulation. Most of the simulation reached steady-state with in second recycle. |
| 저자 | 송성문, 김인호 |
| 소속 | 충남대 |
| 키워드 | IgY; SMB; Aspen |
| 원문파일 | 초록 보기 |
