| 초록 |
Chitosan is the second most plentiful biomass and is already known as a biocompatible, biodegradable, and low-toxic material. Chitosan is also considered to be a good candidate for the gene delivery system, since cationically charged chitosan can be complexed with negatively charged plasmid DNA. Low molecular, water-soluble chitosan (LMWSC, molecular weight of 22kDa) was characterized and evaluated as a gene carrier. The formation of plasmid/LMWSC complex was proved and transfection efficiency in 293T cells was evaluated. The cytotoxicity of LMWSC also was determined by MTT assay. This study showed that LMWSC is one of the candidates for DNA deivery. In this work, artery wall cells targeted gene carrier was synthesized to therapy the disease related with cardiovascular using LMWSC. AWBP-PEG-g-LMWSC was synthesized by conjugation the artery wall binding peptide (AWBP), a specific targeting peptide, to the end of PEG-g-LMWSC. Characters of the synthesized AWBP-PEG-g-LMWSC were analyzed by measuring FT-IR, 1H-NMR, zeta-potentiometer, and AFM. |
