| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 가을 (10/30 ~ 11/01, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
23권 2호 |
| 발표분야 |
포스터_생물공학 |
| 제목 |
Development of MALDI-TOF-MS based quantitative targeted glycomics technique for total N-glycan analysis (MALDI-QTaG) |
| 초록 |
In this study, MALDI-MS based quantitative targeted glycomics (MALDI-QTaG) was developed for monitoring the abnormal glycosylation of clinical and biopharmaceutical products. MALDI-QTaG was proposed for sensitive and quantitative analysis of total N-glycans. The derivatization reactions dramatically increased the linearity and sensitivity relative to underivatized glycans. In addition, the analytical strategy was chromatographic purification-free and non-laborious process accessible to the high-throughput analyses. This MALDI-MS based glycomics technology has wide application in many clinical and bioengineering fields requiring sensitive, quantitative and fast N-glycosylation validation. |
| 저자 |
송원석1, 김윤곤2, 김성민2, 조성현2, 이재승2, 전효진2
|
| 소속 |
1서울대, 2숭실대 |
| 키워드 |
N-glycan; MALDI-MS; UPLC-FLR; Chemical derivatization
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| E-Mail |
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