| 초록 |
Herein, we report the non-genetic modulation of bacterial cellulose (BC) synthesis by Acetobacter xylinum and its application to the layered neuronal tissue emulation. The controlled dispersion of graphene oxide (GO) nanoflakes into culture media not only intervenes the molecular interaction within a cellulose crystalline nanofibril but also modulate the collective association of bacterial bodies, leading to the cellulose nanofibrillar hydrogel with reduced Young’s modulus (~50%) and flattened surface profiles. Furthermore, the real-time investigation of 3D neuronal networks constructed in this GO-incorporated BC hydrogel with broken chiral nematic orderness revealed the vertical locomotion of growth cones, the accelerated neurite outgrowth (~100 μm/day) with reduced backward travel length (~6 μm at Day 1), and the efficient formation of synaptic connectivity with distinct axonal bifurcation points. |
