| 초록 |
Utilization of abundant and cheap carbon sources can effectively reduce the production cost and increase the economic feasibility. In this study, we applied combinatorial approach to further increase the titer of itaconate production from acetate. Initially, the itaconate-specific high-throughput screening system (HTS) could be constructed using recently characterized transcriptional regulator and selection marker. The selective growth was validated in accordance with the addition of itaconate. Thereafter, Adaptive Laboratory Evolution (ALE) was applied to itaconate-producing strain. Evolution was repeated over several rounds and the increase in growth rate was observed under the selection pressure. The evolved strain could produce 2.07 g/L of itaconate, a 1.56-fold increase in comparison with parental strain and the yield could be increased by 1.52-fold. The mutant will be additionally characterized with WGS and rational approach will be applied to maximize the itaconate production. |
