| 초록 |
Introduction of cheap carbon sources are important to production cost and economic feasibility. In the previous study, production of itaconic acid from acetate was introduced with rational metabolic engineering strategy, proving the potential of acetate as a promising carbon source. In this study, we used combinatorial approach to further increase the production of itaconic acid. Initially, the specific screening device was using transcriptional regulator and selection marker. Also, Adaptive Laboratory Evolution (ALE), utilizing natural selection toward microorganisms under selection pressure, was introduced to screen itaconate-producing strain. The evolved strain could produce 1.06 g/L of itaconate, a 1.24-fold increase in comparison with parental strain and the yield was significantly enhanced by 7.16-fold. The rational approach using glyoxylate shunt pathway was used to further increase production which is comparable to the production from glucose as a sole carbon source. |
