| 초록 |
Optically pure amines are important building blocks for pharmaceutical drugs. Omega기호로-Transaminase (Omega기호로-TA) is an attractive enzyme to prepare chiral amines because of its high enantioselectivity and regioselectivity. Here, we prepared optically pure amines by Omega기호로-TA-mediated kinetic resolution of rac-amines using furfural, one of agricultural byproducts from biomass, as an amino acceptor instead of pyruvate. S- and R-selective Omega기호로-TAs from Ochrobactrum anthropi (OATA) and Aspergillus fumigatus (AFTA), respectively, showed high activity toward furfural among six Omega기호로-TAs. Structurally diverse amines were prepared with high enantiopurity (＞99% ee) by kinetic resolution using OATA and AFTA. |
