| 초록 |
When animal cells were culture in air-liquid condition, it shows different metabolism. However, there is rarely studied of development tissue scaffold for air-liquid interface using nanofiber. Therefore we prepared super-hydrophilic nanofiber mesh for a convenient air-liquid cell culture scaffold. Poly (ε-caprolactone)-branched poly (etyleneimine) (PCL-bPEI) electrospun nanofiber (NF) was chemically surface-immobilized with 4-arm poly ethylene glycol (PEG) through surface-exposed amines on NF, layer by layer. As increasing PEG layers from 1 to 5, the thickness of a single fiber was increased 2 times. Super-hydrophilicity was confirmed by water swelling weight ratio, 1200% to PEG layers on NF. Keratinocytes were directly seeded on the surface of the device, cell viability normalized to cell DNA contents was 1.5 times higher with 2 layers of PEG on NF than NF. Therefore, the study focuses on the evaluation ability and influence to air-liquid cell culture in tissue engineering. |
