| 학회 |
한국재료학회 |
| 학술대회 |
2009년 봄 (05/21 ~ 05/22, 무주리조트) |
| 권호 |
15권 1호 |
| 발표분야 |
나노생체재료 |
| 제목 |
The Effect of Silicon Doped Porous BCP Bone Graft Substitutes on the Osteoblast Cell Proliferation, Differentiation and their gene expression |
| 초록 |
Cationic doping of Silicon(Si) within BCP improves the biocompatibility significantly along with functional activity response in the physiological environment of the implant site. Granular bone substitute(BS) with cationic doping was investigated for its effect on the cell proliferation and differentiation. Si was doped during BCP powder synthesis by ultrasonic energy assisted synthesis method. Porous granular bone substitutes were fabricated by fibrous monolithic (FM) process. The biocompatibility of the BS was evaluated by in-vitro study. Mg-63 cellular adhesion, spreading and growth behavior was observed using SEM. Cell differentiation was stimulated with conditioned media(50ug/ml 2-phospho-L-ascorbic acid trisodium salt, 10mM glycerol 2-phosphate disodium salt hydrate) and stained with alizarin red S. The cellular cytotoxicity result showed that cellular viability was better then pure BCP. The cellular adhesion and spreading behavior on graft composites were evident by the presence of very active filopodial growth and microvilli-like projections around the cells. MG-63 cells were also well grown and showed an accelereated differentiation on Si-doped bone substitute. Also the expression of osteoblast specific gene markers(OP, ON, OPG) as well as the phenotypic expression of collagen, MMPs, and TIMPs were observed in this study. |
| 저자 |
In-Seon Byun1, Swapan Kumar Sarkar2, Young-Hee Kim3, Md. Anirban Jyoti4, Young-Ki Min2, Hun-Mo Yang3, Byong-Taek Lee1, Ho-Yeon Song2
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| 소속 |
1Dept. Microbiology, 2School of Medicine, 3Soonchunhyang Univ., 4Dept. Biomedical Engineering and Materials |
| 키워드 |
Silicon-doped bone substitute; Proliferation; Differentiation; MMPs; TIMPs
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| E-Mail |
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