| 초록 |
Syringic acid is a major redox compound obtainable from the degradation of lignin. Whereas the utilization of lignocellulosic biomass has become ever more important to reduce the carbon emission, valorization of lignin has been relatively lag behind compared to cellulose and hemicellulose. Syringic acid can be reduced to syringaldehyde, a value added compound with antioxidant, anticarcinogenic and antibiotic properties. Production of syringaldehyde so far has been dependent on multistep chemical reactions that can be replaced with safer, environment- friendly biological reduction of syringic acid. Herein, the energetically unfavorable reduction was overcome by employing an Escherichia coli whole cell biocatalyst overexpressing a carboxylic acid reductase (CAR). By screening CARs from fourteen different microbial genes, the best-performing enzyme could be selected and the reaction conditions were optimized to achieve a 90% conversion of syringic acid to syringaldehyde. |
