| 학회 |
한국화학공학회 |
| 학술대회 |
2008년 가을 (10/23 ~ 10/24, 부산 BEXCO) |
| 권호 |
14권 2호, p.2768 |
| 발표분야 |
생물화공 |
| 제목 |
HBsAg detection based on SPR and electrochemical assay |
| 초록 |
A way of detecting HBsAg based on surface plasmon resonance and electrochemical assay was demonstrated. HBsAg, together with HBV DNA and anti-HBc, is one of three biomarkers for reliable diagnosis of HBV infection. In order to immobilize the capturing element, anti-HBsAg antibody onto the bare gold surface, fusion protein 6HGBP-ScFv was prepared from Escherichia coli through recombinant protein technology. Thus, the gold surface activated by this kind of protein can be directly used for target HBsAg detection. Both SPR and electrochemical assay exhibited successful interaction between target HBsAg and immobilized antibody fragment [Our work was supported in part by the IT Leading R&D Support Project from the Ministry of Knowledge Economy through IITA and by the KOSEF through the Center for Ultramicrochemical Process Systems.]. |
| 저자 |
Shun Zheng1, 박태정2, 김현욱1, 이상엽2
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| 소속 |
1Dept. of Chemical and Biomolecular Eng., 2KAIST |
| 키워드 |
Hepatitis B virus; surface plasmon resonance; electrochemical detection; biosensor
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| E-Mail |
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| 원문파일 |
초록 보기 |
