| 초록 |
Signal transducer and activator of transcription 3 (STAT3) protein is well-known cytoplasmic transcriptions factor that regulatory functions in cell proliferation, differentiation and survival. The STAT3 proteins are phosphorylated at tyrosine residue by tyrosine kinases. It promotes phosphorylated STAT3 monomers to form dimers and translocate to nuclear and binding to specific DNA, gene transcription is activated. In many cancers, STAT3 protein found over-activated form and it lead to uncontrolled expression of the STAT3 target genes. Here, we first demonstrate the tracking of STAT3 signaling pathway such as phosphorylation, dimerization of STAT3 protein based on localized surface plasmon resonance (LSPR) spectra. Dramatic LSPR Δλmax shifts were measured in the STAT3 signaling pathway steps and these signals were reduced by the treatment of inhibitor of STAT3 dimer. These results suggest that our system is able to effectively applicate for drug discovery for anti-cancer therapy. |
