| 학회 |
한국공업화학회 |
| 학술대회 |
2004년 가을 (10/29 ~ 10/30, 호서대학교(아산캠퍼스)) |
| 권호 |
8권 2호 |
| 발표분야 |
생물공학 |
| 제목 |
An Unique Iterative Type I PKS, Neocarzinostatin Naphthoate Synthase: Analysis and swapping of domains with AviM |
| 초록 |
An enediyne antibiotic, Neocarzinostatin (NCS), produced by Streptomyces carzinostaticus is a potent anti-cancer antibiotic and is structurally constituted with warhead diene, the naphthoic acid moiety and a sugar moiety. The NNS was heterologously expressed in Streptomyces hosts and the product was isolated and identified as 2-hydroxy-5-methyl-1-naphthoic acid (major) along with minor 2, 7-dihydroxy-5-methyl-1-naphthoic acid. Taking into an account the isolation of bicyclic aromatic compound and the monocyclic compound from the bacterial PKSs, which in turn have similar domain organizations, the chain length factor can be analyzed by swapping of domains to observe the expected hybrid compound. The KS and KS/ACP domains from bicyclic aromatic compound producer Streptomyces carzinostaticus and monocyclic aromatic compound producer Streptomyces viridochromogenes Tü 57 have been swapped to establish the factor. |
| 저자 |
Devi Bahadur basnet, Basundhara Sthapit, 이희찬, 류광경, 송재경
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| 소속 |
선문대 |
| 키워드 |
Neocarzinostatin; Neocarzinostatin Naphthoate Synthase (NNS); domain swapping.
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| E-Mail |
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