| 학회 |
한국화학공학회 |
| 학술대회 |
2013년 봄 (04/24 ~ 04/26, 광주 김대중컨벤션센터) |
| 권호 |
19권 1호, p.347 |
| 발표분야 |
생물화공 |
| 제목 |
Carbonic anhydrase: Biocatalytic agent for CO2 capture system development |
| 초록 |
Carbonic anhydrase (CA, EC 4.2.1.1) is one of the enzymes, which can be employed for CO2 sequestration technology development. CA catalyzes a reversible hydration of carbon dioxide: CO2 + H2O ↔ H+ + HCO3- (1.1) (Box 1) including varieties of other reactions. Interestingly, CA has the ability to catalyze the hydration of over 600,000 molecules of carbon dioxide per molecule of CA per second comparable to a theoretical maximum rate of 1,400,000. CA can fix large quantities of CO2 into CaCO3 in presence of suitable cations at modest pH values in vitro. CA has such distinctive CO2-catalyzing properties and is now being attended as prominent biocatalysts for CO2 sequestration technology development. Here, new genes of functional alpha type-CAs (aCA) from marine resources were acquired and synthesized as codon-optimized forms for E. coli expression. We set up over-expression system of aCA in E. coli. host and prepare separation/purification (renaturation, if necessary) procedures to produce high-functional CA forms. |
| 저자 |
백승필, 민기하, Bashistha Kumar KANTH, 유영하, 장의경, 여기백, 기미란
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| 소속 |
고려대 |
| 키워드 |
marine resource; carbonic anhydrase; CO2 capture
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| E-Mail |
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| 원문파일 |
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