| 학회 |
한국공업화학회 |
| 학술대회 |
2015년 봄 (04/29 ~ 05/01, BEXCO (부산)) |
| 권호 |
19권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Modulation of protein folding rate of GFP through beta-turn engineering |
| 초록 |
Control of the protein folding rate is very important in various biological studies and biotechnology. The present study examined the possibility of modulating the protein folding rate through beta-turn engineering of green fluorescence protein (GFP). A type II’ two residue beta-turn in GFP was targeted to generate various sets of mutants. The design of mutants was based on the rationale that residues i+1 and i+2 of a beta-turn have defined residue preferences, and their perturbation affects the rate of protein folding. The in vitro refolding kinetics performed with purified variants showed the variantion of GFP folding rate as expected. This kind of study would be useful in the modulation of in vivo activities of recombinant proteins which have been generally controlled at transcription and translation levels. |
| 저자 |
Bharat Madan, Govindan Raghunathan, Sriram Sokalingam, Thangappan Jayaraman, Sungwan Park, 이선구
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| 소속 |
부산대 |
| 키워드 |
protein folding; protein engineering; beta-turn
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| E-Mail |
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