| 학회 | 한국고분자학회 |
| 학술대회 | 2004년 가을 (10/08 ~ 10/09, 경북대학교) |
| 권호 | 29권 2호, p.355 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Neurogenesis of Bone Marrow Stromal Cell onto Butylated Hydroxyanisole / Poly(L-lactide-co-glycolide) Films |
| 초록 | It has been widely recognized that bone marrow stromal cells (BMSCs) can be differentiated into osteoblasts, chondrocytes, and neuronal cells under the controlling of culture condition.1,2 In the present study, poly(L-lactide-co-glycolide) film was fabricated to release butylated hydroxyanisole (BHA) for the purpose of neurogenesis of BMSCs. BHA loaded PLGA films manufactured as the concentrations of 200, 400, and 600 μM/0.2 g PLGA and characterized by scanning electron microscope (SEM). The inverted microscope used for the observation of the morphology of BMSCs which is cultivated on BHA/PLGA film during 5 days. MTT assay used for the measurement of the viability of the cells and cytotoxicity. After 5 days, it could be confirmed that BMSCs were differentiated into neuronal cells by means of staining such as neurofilament (NF) and neuron-specific enolase (NSE). The morphology of the fabricated PLGA film throughly revealed uniform layers without pore structure. The changes of BMSCs to neuronal cell observed onto the fabricated PLGA film from after 1 day by the stimulation of BHA. The more the concentration of BHA increased, the less the viability of the cells, however, the cytotoxicity of the cells showed little effect until up to 600 μM/0.2 g PLGA of concentration from the result of immunohistochemistry. It can be concluded that the controlled release of BHA from BHA loaded PLGA film might be differentiated BMSCs to neuronal cells due to the diffusion of BHA and biodegradation of PLGA. This study suggested that BHA/PLGA film is released the desired period in delivery system and useful neuronal growth culture of BMSCs for the application of neuronal regenerative medicine. Fig. 1. Schematic diagram of fabrication method for BHA/PLGA film. Acknowledgments : This work was supported by Korea Research Foundation (KRF) (2003-041-D00209) References 1. E. M. Horwitz, D. J. Prockop, L. A. Fitzpartick, W. W. Koo, P. L. Gordonm, M. Neel, M. Sussman, P. Orchard, J. C. Marx, R. E. Pyeritz, and M. K. Brenner, Nature Med., 5, 309 (1999). 2. I. Lee, J. H. Yang, J. S. Y., H. J. Lee, J. K. Kang, M. C. Kim, G. Khang, S. J. Lee, and H. B. Lee, Macromol. Chem. Symp., 15(4), 227-249 |
| 저자 | 장우영1, 김선화1, 이일우2, 이종문1, 강길선1 |
| 소속 | 1전북대, 2대전가톨릭의대 신경외과 |
| 키워드 | tissue engineering; BHA; PLGA film; nerve regeneration |
