| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 가을 (10/13 ~ 10/14, 제주 ICC) |
| 권호 | 30권 2호 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | The using of PLGA scaffold included SIS for Osteogenesis |
| 초록 | Bone marrow stromal cells (BMSCs) have pluripotent differentiation potential including differentiation into osteoblasts, chondrocytes, adipocytes, and muscle cells.1 Meanwhile small interimal submucosa (SIS) is a naturally occurring tissue matrix composed of extracellular matrix proteins and various growth factor. SIS is easily derived from the porcine jejunum. therefore, SIS have the potential in the biomedical application as the scaffold. It was confirmed that BMSC is stem cell by flow cytometry. In this work, we examined the differentiationof rat bone marrow stem cells (rBMSC) into osteoblasts on SIS/PLGA scaffold in the presence of osteogenic media. rBMSCs were supplemented with DMEM and 10% FBS, cultured for up to passage 5. Then the rBMSCs were cultured on PLGA (90,000 g/mole). For in vitro osteogenesis, rBMSCs were supplemented with osteogenic medium. The osteogenic medium consisted of DMEM, 10mM β-glycerophosphate, 50 μm/ml L-ascorbic acid, 10-7 M dexamethasone, 10-8 M vitamin D3, 10% FBS and 1% penicillin-streptomycin. While, control medium consisted of DMEM, 10% FBS and 1% penicillin-streptomycin. rBMSCs on scaffold maintained for 7, 14, 21, and 28 days. Osteogenic differentition in vitro was evaluated by means of MTT, ALP activity and PCR. Cell viability on scaffold increase as culture day increased and in addition, ALP activity increased. the result of these experiments indicated that rBMSCs differentiated into osteoblasts. Acknowledgement : This work was supported by KMOHW(0405-BO01-0204-0006) 1. F. Sugiura, H. Kitoh, and N. Ishiguro, B. B. R. C. 316, 233 (2004). |
| 저자 | 이창래1, 김순희1, 김문석2, 이종문1, 이해방2, 강길선1 |
| 소속 | 1전북대, 2한국화학(연) |
| 키워드 | osteogenesis; BMSCs; SIS |
