| 학회 |
한국공업화학회 |
| 학술대회 |
2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
26권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
A rapid and sensitive strategy for the detection of long RNA targets via invasive cleavage reaction and toehold-mediated rolling circle amplification |
| 초록 |
An intriguing combination of DNA-processing enzymes enables them to transform into a new generation of molecular diagnostic tools. Here, we report an isothermal amplification strategy by co-employing a structure-specific endonuclease (flap endonuclease 1, FEN1) and a strand displacing-DNA polymerase (Bst DNA polymerase) to detect long RNA targets. This approach couples FEN1-driven invasive cleavage reaction with toehold-mediated rolling circle amplification (iFEN-tRCA), allowing for highly selective and sensitive detection of long RNA targets and offering a detection limit below 10 pM within 1 hr. By using human epidermal growth factor receptor 2 (HER2, encoded by ERBB2) as a target, we demonstrate the feasibility and quantitative capability of the iFEN-tRCA assay to determine ERBB2 mRNA levels in human breast cancer cells. Therefore, we envision that the developed assay could provide a suitable molecular diagnostic tool. |
| 저자 |
김다인1, 이지영1, 임재우2, 임은경2, 김은정1
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| 소속 |
1인천대, 2한국생명공학(연) |
| 키워드 |
rolling circle amplification |
| E-Mail |
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