| 초록 |
Cavitation extraction, water/hexane washing, and cavitation fractional precipitation methods were developed to efficiently recover and purify paclitaxel from biomass. The paclitaxel yield was 94-100% in ultrasound-negative pressure cavitation extraction. This investigation of the extraction mechanism proved that cavitation bubbles themselves play a key role in promoting cell disruption, which has been indicated as improving the recovery efficiency of paclitaxel. In addition, a high purity of paclitaxel was obtained in a short operating time by sequential washing with water and hexane. Furthermore, when paclitaxel was precipitated under a negative pressure of -200 mmHg, almost all of the paclitaxel (>99.9%) could be recovered in a short operation time (5 min). As a result of investigating the mechanism of fractional precipitation in which cavitation bubbles were introduced, it was found that the bubble surface itself acts as a nucleation site, resulting in faster nucleation and thereby improving precipitation efficiency. [This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Government of Korea (MSIT) (Grant Number: 2021R1A2C1003186)] |
