| 초록 |
Formate is the simplest organic acid and reported as one of the desirable bulk chemicals regarding economic and environmental benefits. However, lots of microorganisms cannot use formate because of its toxicity and related enzymes. In this study, we used Discovery Studio with CHARMm, a molecular dynamics engine, for finding candidates of enzyme engineering. We hypothesized that the regulation of structure in the enzyme level could improve substrate uptake. First, we can build a suitable homology model based on the known sequence and structure. Using the constructed model, we are able to calculate CDOCKER Energy for estimating the stability of the enzyme when the enzyme binds to the substrate. According to the serial results, we can find the appropriate mutant candidates for improving enzyme stability. Finally, the modified enzyme structure might be show improvement of formate uptake. Based on the results, we might get a great mutant to improving formate uptake as a sole carbon source. |
