| 학회 |
한국화학공학회 |
| 학술대회 |
2007년 봄 (04/19 ~ 04/20, 울산 롯데호텔) |
| 권호 |
13권 1호, p.443 |
| 발표분야 |
생물화공 |
| 제목 |
Functional expression of CalB(Candida antarctica lipase B) in P. pastoris and its application for the synthesis of glycerol carbonate |
| 초록 |
Candida antarctica lipase B (CalB) is an efficient biocatalyst for many organic synthesis reactions. We found that CalB catalyzed the synthesis of glycerol carbonate from glycerol and dimethyl carbonate through transesterification. Glycerol carbonate is a useful compound as solvent, additive, monomer, and chemical intermediate. Glycerol is produced as by-product in large amounts during the production of biodiesel. To obtain the optimized CalB for the synthesis of glycerol carbonate, recombinant CalB was expressed in the methylotropic yeast Pichia pastoris. In addition, CalB were immobilized on PMMA macroporous carrier supports for the synthesis of glycerol carbonate. |
| 저자 |
김현숙1, 박혜정1, 정광섭2, 김상철3, 김용환1
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| 소속 |
1광운대, 2GS칼텍스, 3한국화학(연) |
| 키워드 |
lipase; CalB; P.pastoris; gylcerol carbonate
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| E-Mail |
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| 원문파일 |
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