| 학회 |
한국화학공학회 |
| 학술대회 |
2008년 가을 (10/23 ~ 10/24, 부산 BEXCO) |
| 권호 |
14권 2호, p.2731 |
| 발표분야 |
생물화공 |
| 제목 |
Functional expression ofCandida antarctica lipase B (CalB) in Escherichia coli |
| 초록 |
Candida antarctica Lipase B (CalB) was functionally expressed in the cytoplasm of Escherichia coli Origami(DE3) with the N-terminus fusion of E. coli endogenous proteins. The previously-identified stress responsive proteins dramatically increased the solubility of CalB in E. coli cytoplasm when used as N-terminus fusion partners. We demonstrated thatthese stress responsive proteinswere powerful solubility enhancers that presumably facilitated the protein folding of CalB. Moreover, one of the fusion mutant showed the highest hydrolytic activity and was as biologically active as standard CalB. Similarly to the previous report, the electrophoretic properties of CalB indicate that CalB seems to form dimer-based oligomer structures. We evaluated the structural compatibility between the fusion partner protein and CalB, which seems to be of crucial importance upon the bioactive dimer formation of CalB and might affect the substrate accessibility to the enzyme active site, thereby determining the biological activities of the fusion mutants. |
| 저자 |
서혁성, 김성은, 한경연, 박진승, 송종암, 안금영, 이종환, 이은정, 권수정, 신재욱, 이지원
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| 소속 |
고려대 |
| 키워드 |
CalB; functional expression; fusion mutant; bioactivity; Escherichia coli
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| E-Mail |
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| 원문파일 |
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