화학공학소재연구정보센터
학회 한국공업화학회
학술대회 2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU))
권호 26권 1호
발표분야 포스터-생물공학
제목 Reporter engineering for the enhancedCRISPR/Cas trans-cleavage activity and sensitive biomolecular assay​
초록 Recently, trans-cleavage activity has beendiscovered in some Cas proteins (e.g., Cas12a or Cas13a), and various detectionmethods using this activity have been reported in many studies. In this work,we developed a highly efficient DNA reporter (5’-TTATT-CCCCC-3’) that surpassedthe performance of the existing AT-rich DNA reporter (5’-TTATT-3’). Bysystematically investigating the effect of length and sequence of DNA reporterson the trans-cleavage activity of Cas12a, we achieved up to 10-fold increase insignal intensities compared to existing AT-rich DNA reporter. The new DNAreporter was also applied for the detection of Salmonella STN gene.Importantly, both detection speed and limit were significantly enhanced withthe new DNA reporter.
저자 이승진, 남대한, 박기수
소속 건국대
키워드 CRISPR-Cas; Cas12a; reporter;
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