초록 |
Recently, trans-cleavage activity has beendiscovered in some Cas proteins (e.g., Cas12a or Cas13a), and various detectionmethods using this activity have been reported in many studies. In this work,we developed a highly efficient DNA reporter (5’-TTATT-CCCCC-3’) that surpassedthe performance of the existing AT-rich DNA reporter (5’-TTATT-3’). Bysystematically investigating the effect of length and sequence of DNA reporterson the trans-cleavage activity of Cas12a, we achieved up to 10-fold increase insignal intensities compared to existing AT-rich DNA reporter. The new DNAreporter was also applied for the detection of Salmonella STN gene.Importantly, both detection speed and limit were significantly enhanced withthe new DNA reporter. |