| 학회 |
한국고분자학회 |
| 학술대회 |
2010년 봄 (04/08 ~ 04/09, 대전컨벤션센터) |
| 권호 |
35권 1호 |
| 발표분야 |
세포기능조절 고분자(의료용 고분자 부문위원회) |
| 제목 |
Hydrogel microwell arrays for directing embryonic stem cell fate |
| 초록 |
Directed embryonic stem (ES) cell differentiation is a powerful approach for generating a renewable source of cells for regenerative medicine. Typical ES cell differentiation protocols involve the formation of ES cell aggregate intermediates called embryoid bodies (EBs). Recently, we have demonstrated the use of poly(ethylene glycol) microwell arrays as templates for directing the formation of these aggregates, offering control over parameters. We improved the platform by optimizing a number of features: material composition of the microwells, aggregate retrieval methods. Adopting these modifications, we demonstrated an improved degree of homogeneity of the resulting aggregate populations and established a robust protocol for eliciting high EB formation efficiencies. Furthermore, we demonstrated the EB size-dependent cardiac and endothelial cell differentiation. Therefore, the development of hydrogel microwell arrays will help in advancing our understanding of stem cell biology. |
| 저자 |
정봉근1, 황유식2, Daniel Ortmann3, Nobuaki Hattori3, Hannes-Christian Moeller3, Ali Khademhosseini3
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| 소속 |
1한양대, 2경희대, 3하버드 의대 |
| 키워드 |
Hydrogel microwell; stem cell differentiation
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| E-Mail |
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