| 초록 |
The SEA (specific enzymatic activities) of sMMO are widely investigated to understand its enzymatic activity and turnover rate to understand the influence by CH4, O2, e-, and H+. The ratio between substrates and enzymes need to be further investigated to understand its possible influences during expression of sMMO. The expression of MMOH is described based on the ratio of CH4 and O2. The expression level in sMMO is important for the purification of MMOH and the complex formation of six subunits needs to discover further mechanism studies through MMOG. The CH4 oxidation is performed in α-subunit of MMOH and β-subunit generates specific interactions for the dimeric formation of MMOH. The function of γ-subunit is not well quite understood, but this small-subunit only detected in water-soluble region after cell-lysis. The CH4 concentration of sMMO proved that the injection volume of CH4 and O2 affect the volume of soluble portion of MMOH. In addition, the differences of these species depend on the concentration of γ-subunit. The SEA are affected by the concentration of CH4 and O2, the ratio of reductase components. Also, high concentration of O2 decrease the SEA. |
