| 초록 |
In vitro development of highly-organized three dimensional (3D)-engineered tissues consist of multiple types of cells and ECM, which possess a similar structure and function as natural tissues, is a key challenge for tissue engineering and pharmaceutical assay. We developed a simple and unique bottom-up approach, "hierarchical cell manipulation", using nanometer-sized Layer-by-Layer films consisting of fibronectin and gelatin (FN-G) as a nano-extracellular matrix. The FN-G nanofilms were prepared directly on the cell surface, and we discovered that at least 6 nm thick FN-G films acted as a stable adhesive surface for adhesion of the second cell layer. We have also developed a rapid bottom-up approach, "cell-accumulation technique", by a single cell coating using FN-G nanofilms. This rapid approach easily provided more than twenty-layered 3D-tissues with blood capillary networks after only one day of incubation. Both manipulations will be promising to achieve organ on a chip. |
