| 초록 |
Recently, the development of three-dimensional (3D) cell spheroid systems is considered as a promising technology to mimic in vivo microenvironment. The 3D-cultured cells reflect better in vivo characteristics compared to monolayer (2D)-cultured cells. However, limited size of spheroid due to the insufficient supply of oxygen, nutrients into the central region of the spheroid is still remained as a critical huddle. In this study, we developed novel microparticles with leaf-stacked structure throughout entire particles (ELSS particles) which can allow sustained release of bioactive molecules as well as provide space for sufficient supply of oxygen, nutrients in cell spheroids. The cell spheroid system was prepared using the ELSS particles and human bone marrow-derived mesenchymal stem cells (hBMSCs). The morphology of ELSS particles, BMP-2 release pattern from ELSS particles, cell survival and osteogenic differentiation of the cell spheroid hybrid system were investigated. |
