| 학회 |
한국공업화학회 |
| 학술대회 |
2015년 봄 (04/29 ~ 05/01, BEXCO (부산)) |
| 권호 |
19권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Development of New Anchoring motif, yuaB, for Bacillus subtilis Cell Surface Display |
| 초록 |
To develop new Bacillus subtilis cell surface display system, we used YuaB as an anchoring motif. YuaB is the major component of Bacillus subtilis biofilm. YuaB is located in B. subtilis cytoplasmic membrane and plays role of making repellency of B. subtilis biofilm after cell clusters are formed. We fused His6 tag at C-terminal YuaB to comfirm surface display and used this for heavy metal adsorption. We confirmed that YuaB can be used as an anchoring motif by flow cytometry. AA analysis result shows that His6 tag has activities for heavy metal adsorption on the B. subtilis surface. |
| 저자 |
김준형 |
| 소속 |
동아대 |
| 키워드 |
Bacillus subtilis; surface display; yuaB
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| E-Mail |
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