| 초록 |
In this study, we prepared cell-derived matrices (CDMs) from in vitro cultured cells and evaluated their effects as a physical cue in the induction of osteogenic differentiation of MC3T3 preosteoblasts. Poly(lactic-co-glycolic) acid (PLGA) films were fabricated by using solvent evaporation technique. Each cell type was seeded on PLGA films, and cultured for 1 week, then decellularized using a cocktail solution of detergents and enzymes. The composition of CDM deposited on the films was examined using immunofluorescence of fibronectin, laminin, and type I collagen. Several substrates were tested: control (PLGA film), gelatin-coated film, fibroblast-derived matrix (FDM), preosteoblast-derived matrix (PDM), and chondrocyte-derived matrix (CHDM). After preosteoblasts seeding, cell morphology and proliferation were investigated using SEM and CCK-8 assay, respectively. Improved mineralization and calcium deposition in the CDMs were confirmed using Von kossa and alizarin red S staining. |
